Recombinant expression, purification and characterization of human soluble tumor necrosis factor receptor 2.

Li, An; Sun, Kuo; Wang, Junjie; Wang, Shuocun; Zhao, Xin; Liu, Runhui; Lu, Yiming

Li, An; Sun, Kuo; Wang, Junjie; Wang, Shuocun; Zhao, Xin; Liu, Runhui; Lu, Yiming.

Afiliação

Li A; School of Pharmacy, Second Military Medical University, Shanghai, 200433, China; Department of Critical Care Medicine, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, 200072, China.
Sun K; School of Pharmacy, Second Military Medical University, Shanghai, 200433, China.
Wang J; Department of Critical Care Medicine, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, 200072, China.
Wang S; School of Medicine, Shanghai University, Shanghai, 200444, China.
Zhao X; Department of Critical Care Medicine, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, 200072, China.
Liu R; School of Pharmacy, Second Military Medical University, Shanghai, 200433, China. Electronic address: lyliurh@126.com.
Lu Y; School of Pharmacy, Second Military Medical University, Shanghai, 200433, China; Department of Critical Care Medicine, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, 200072, China; School of Medicine, Shanghai University, Shanghai, 200444, China. Electronic addres

Protein Expr Purif ; 182: 105857, 2021 06.

Article em En | MEDLINE | ID: mdl-33639277

Assuntos

Escherichia coli; Expressão Gênica; Receptores Tipo II do Fator de Necrose Tumoral; Escherichia coli/genética; Escherichia coli/metabolismo; Humanos; Receptores Tipo II do Fator de Necrose Tumoral/biossíntese; Receptores Tipo II do Fator de Necrose Tumoral/química; Receptores Tipo II do Fator de Necrose Tumoral/genética; Receptores Tipo II do Fator de Necrose Tumoral/isolamento & purificação; Proteínas Recombinantes/biossíntese; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Proteínas Recombinantes/isolamento & purificação; Solubilidade

Palavras-chave

Anti-tumor immunotherapy; Inclusion body; Prokaryotic expression; Protein refolding; TNF receptor

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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Expressão Gênica / Receptores Tipo II do Fator de Necrose Tumoral / Escherichia coli Limite: Humans Idioma: En Revista: Protein Expr Purif Ano de publicação: 2021 Tipo de documento: Article

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