Characterization of exopolysaccharides produced by Weissella confusa XG-3 and their potential biotechnological applications.
Int J Biol Macromol
; 178: 306-315, 2021 May 01.
Article
em En
| MEDLINE
| ID: mdl-33652047
ABSTRACT
In this study, exopolysaccharides (EPSs) produced by Weissella confusa XG-3 were characterized. The monosaccharide composition of XG-3 EPS was determined to include glucose according to GC data, and its molecular weight was 3.19 × 106 Da, as determined by HPLC. Scanning electron microscopy (SEM) revealed a smooth, porous, and branched structure, and atomic force microscopy (AFM) confirmed the presence of round lumps and chains on irregular surfaces of XG-3 EPS. The results of the Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) analyses suggested that XG-3 EPS is a linear α-(1,6)-linked dextran. X-ray diffraction (XRD) data confirmed the noncrystalline amorphous structure, and the results of the Congo red assay corresponded to the random coiled chain conformation of XG-3 dextran. XG-3 dextran exhibited good radical scavenging activity and reducing power and possessed high thermal stability, with a degradation temperature (Td) of 306.8 °C. The absolute value of the zeta potential and particle size of XG-3 dextran continually increased with increasing dextran concentration. The water contact angle showed that XG-3 dextran had relatively high hydrophobicity in the presence of sucrose. XG-3 dextran stimulated the growth of Lactobacillus spp. and Bifidobacterium spp. These findings indicate that XG-3 dextran has unique characteristics and can be potentially applied as a food additive and an antioxidant.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos Bacterianos
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Sequestradores de Radicais Livres
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Dextranos
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Weissella
Idioma:
En
Revista:
Int J Biol Macromol
Ano de publicação:
2021
Tipo de documento:
Article