Expression and Purification of Functionally Active Serotonin 5-HT<sub>2A</sub> Receptor in Insect Cells Using Low-titer Viral Stock.

Mozumder, Sukanya; Mahesh, Gopa; Srinivasan, Krishnamoorthi; Sengupta, Jayati; Mukherjee, Sujoy

Expression and Purification of Functionally Active Serotonin 5-HT_2A Receptor in Insect Cells Using Low-titer Viral Stock.

Mozumder, Sukanya; Mahesh, Gopa; Srinivasan, Krishnamoorthi; Sengupta, Jayati; Mukherjee, Sujoy.

Afiliação

Mozumder S; Structural Biology and Bioinformatics Division, CSIR Indian Institute of Chemical Biology, Kolkata, India.
Mahesh G; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
Srinivasan K; Structural Biology and Bioinformatics Division, CSIR Indian Institute of Chemical Biology, Kolkata, India.
Sengupta J; Structural Biology and Bioinformatics Division, CSIR Indian Institute of Chemical Biology, Kolkata, India.
Mukherjee S; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.

Bio Protoc ; 10(15): e3704, 2020 Aug 05.

Article em En | MEDLINE | ID: mdl-33659368

Palavras-chave

Serotonin 5-HT2A receptor; GPCR production; Glycosylation; Low MOI expression; Sf9 cell culture; baculoviral expression

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Bio Protoc Ano de publicação: 2020 Tipo de documento: Article

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Bio Protoc Ano de publicação: 2020 Tipo de documento: Article