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Determination of maytansinoids in Trewia nudiflora using QuEChERS extraction combined with HPLC.
Li, Ya-Nan; Li, Jia-Nan; Ouyang, Qin; Zhou, Yu-Bo; Lei, Chun; Cui, Ming-Jun; Fu, Kai-Cong; Li, Jia; Huang, Jian-Ming; Hou, Ai-Jun.
Afiliação
  • Li YN; School of Pharmacy, Fudan University, Shanghai 201203, China.
  • Li JN; Open Studio for Druggability Research of Marine Natural Products, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China; National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
  • Ouyang Q; School of Pharmacy, Fudan University, Shanghai 201203, China.
  • Zhou YB; Open Studio for Druggability Research of Marine Natural Products, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China; National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
  • Lei C; School of Pharmacy, Fudan University, Shanghai 201203, China.
  • Cui MJ; Puer Institute of Traditional Ethnomedicine, Yunnan 665000, China.
  • Fu KC; Puer Institute of Traditional Ethnomedicine, Yunnan 665000, China.
  • Li J; Open Studio for Druggability Research of Marine Natural Products, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China; National Center for Drug Screening, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences
  • Huang JM; School of Pharmacy, Fudan University, Shanghai 201203, China. Electronic address: jmhuang@shmu.edu.cn.
  • Hou AJ; School of Pharmacy, Fudan University, Shanghai 201203, China. Electronic address: ajhou@shmu.edu.cn.
J Pharm Biomed Anal ; 198: 113993, 2021 May 10.
Article em En | MEDLINE | ID: mdl-33677280
ABSTRACT
Three maytansinoids with strong cytotoxicities, dehydrotrewiasine, maytanbutine, and trewiasine, were isolated and identified from Trewia nudiflora, and maytanbutine was obtained from this plant for the first time. A quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction combined with high-performance liquid chromatography (HPLC) was established to determine the three maytansinoids in T. nudiflora. The effects of major factors on the extraction efficiency of the QuEChERS method were evaluated and the optimal conditions using acetonitrile-ethyl acetate (11, v/v) as the extraction solvent and PestiCarb as the clean-up sorbents were established. Compared with Soxhlet extraction (SE) and ultrasonic-assisted extraction (UAE), the QuEChERS method was easy-to-operate and afforded a cleaner extract. A phenomenex HyperClone BDS C18 column was used for HPLC analysis. Methanol-acetonitrile-water was chosen as mobile phase for gradient elution. Method validation showed that all analytes showed good linearity (r > 0.999) over the investigated ranges and satisfactory recoveries ranging from 95.0% to 105.0%. The developed QuEChERS-HPLC method was simple, efficient, and applicable to the determination of maytansinoids in T. nudiflora.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Água / Espectrometria de Massas em Tandem Tipo de estudo: Prognostic_studies Idioma: En Revista: J Pharm Biomed Anal Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Água / Espectrometria de Massas em Tandem Tipo de estudo: Prognostic_studies Idioma: En Revista: J Pharm Biomed Anal Ano de publicação: 2021 Tipo de documento: Article