Development of a novel capillary electrophoresis-based approach for detection of anti-drug antibody responses.
J Immunol Methods
; 494: 113047, 2021 07.
Article
em En
| MEDLINE
| ID: mdl-33775673
ABSTRACT
Peggy Sue is a capillary-based western/immunoassay platform that can separate and characterize proteins by size or charge. A quick and automated immunogenicity assay was developed on Peggy Sue based on charge separation and compared with a traditional bridging method using preclinical samples from non-human primate studies. The results generated with the Peggy Sue assay were comparable to those of the bridging assays. The Peggy Sue platform has several advantages, including time efficiency, low sample consumption, and easy automation. The platform is especially ideal for further characterization of anti-drug antibody (ADA) specificity against complex biologics such as bispecific or multi-specific biotherapeutics as it is easy to conduct domain specificity assessment of observed ADA responses. Our evaluation suggests that the Peggy Sue platform is a promising tool for preclinical ADA analysis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Produtos Biológicos
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Imunoensaio
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Efeitos Colaterais e Reações Adversas Relacionados a Medicamentos
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Anticorpos de Domínio Único
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Anticorpos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
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Humans
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
2021
Tipo de documento:
Article