Protocol for high-throughput compound screening using flow cytometry in THP-1 cells.
STAR Protoc
; 2(2): 100400, 2021 06 18.
Article
em En
| MEDLINE
| ID: mdl-33778785
ABSTRACT
Flow cytometry is a valuable method for analyzing protein expressions at the single cell level but can be difficult to apply to large numbers of samples. This protocol provides instructions to perform a high-throughput small molecule screen using flow cytometry analysis of THP-1 cells, a human monocytic leukemia cell line. We describe a methodology for identifying compounds that regulate PD-L1 surface expression in IFN-γ-stimulated cells, which has been successfully used to screen a collection of â¼200,000 compounds. For complete details on the use and execution of this protocol, please refer to Zavareh et al. (2020).
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Avaliação Pré-Clínica de Medicamentos
/
Ensaios de Triagem em Larga Escala
/
Citometria de Fluxo
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Humans
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2021
Tipo de documento:
Article