Using methylcholanthrene-induced fibrosarcomas to study tumor immunology.
Methods Cell Biol
; 163: 59-75, 2021.
Article
em En
| MEDLINE
| ID: mdl-33785169
ABSTRACT
Mouse models of cancer are essential in furthering our understanding both of the mechanisms that drive tumor development and the immune response that develops in parallel, and also in providing a platform for testing novel anti-cancer therapies. The majority of solid tumor models available rely on the injection of existing cancer cell lines into naïve hosts which, while providing quick and reproducible model systems, typically lack the development of a tumor microenvironment that recapitulates those seen in human cancers. Administration of the carcinogen 3-methylcholanthrene (MCA), allows tumors to develop in situ, forming a tumor microenvironment with an established stroma and vasculature. This article provides a detailed set of protocols for the administration of MCA into mice and the subsequent monitoring of tumors. Protocols are also provided for some of the routinely used downstream applications that can be used for MCA tumors.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fibrossarcoma
/
Metilcolantreno
Tipo de estudo:
Guideline
Limite:
Animals
Idioma:
En
Revista:
Methods Cell Biol
Ano de publicação:
2021
Tipo de documento:
Article