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Analysis of Glycoproteins by ATR-FTIR Spectroscopy: Comparative Assessment.
Derenne, Allison; Derfoufi, Kheiro-Mouna; Cowper, Ben; Delporte, Cédric; Butré, Claire I; Goormaghtigh, Erik.
Afiliação
  • Derenne A; Center for Structural Biology and Bioinformatics, Laboratory for the Structure and Function of Biological Membranes, Campus Plaine CP206/02, Université Libre de Bruxelles, Brussels, Belgium. alderenn@ulb.ac.be.
  • Derfoufi KM; Center for Structural Biology and Bioinformatics, Laboratory for the Structure and Function of Biological Membranes, Campus Plaine CP206/02, Université Libre de Bruxelles, Brussels, Belgium.
  • Cowper B; National Institute for Biological standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, UK.
  • Delporte C; Pharmacognosy, Bioanalysis and Drug Discovery and Analytical Platform, RD3 Department, Faculty of Pharmacy, Université libre de Bruxelles, Brussels, Belgium.
  • Butré CI; Quality Assistance, Donstiennes, Belgium.
  • Goormaghtigh E; Center for Structural Biology and Bioinformatics, Laboratory for the Structure and Function of Biological Membranes, Campus Plaine CP206/02, Université Libre de Bruxelles, Brussels, Belgium.
Methods Mol Biol ; 2271: 361-374, 2021.
Article em En | MEDLINE | ID: mdl-33908020
ABSTRACT
FTIR spectroscopy has been widely used to characterize biopharmaceuticals for many years, in particular to analyze protein structure. More recently, it was demonstrated to be a useful tool to study and compare protein samples in terms of glycosylation. Based on a spectral region specific to carbohydrate absorption, we present here a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition. This FTIR information is compared to MS information. Both approaches yield consistent results but it appears FTIR analysis is easier and more rapid to perform comparisons.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Glicoproteínas / Processamento de Proteína Pós-Traducional / Espectroscopia de Infravermelho com Transformada de Fourier / Anticorpos Monoclonais Tipo de estudo: Prognostic_studies Idioma: En Revista: Methods Mol Biol Ano de publicação: 2021 Tipo de documento: Article
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Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Glicoproteínas / Processamento de Proteína Pós-Traducional / Espectroscopia de Infravermelho com Transformada de Fourier / Anticorpos Monoclonais Tipo de estudo: Prognostic_studies Idioma: En Revista: Methods Mol Biol Ano de publicação: 2021 Tipo de documento: Article