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Databases for technical aspects of immunohistochemistry: 2021 update.
Moroki, Takayasu; Matsuo, Saori; Hatakeyama, Hirofumi; Hayashi, Seigo; Matsumoto, Izumi; Suzuki, Satoshi; Kotera, Takashi; Kumagai, Kazuyoshi; Ozaki, Kiyokazu.
Afiliação
  • Moroki T; Conference on Experimental Animal Histopathology.
  • Matsuo S; Department of Safety Research, Drug Safety Research, Tokushima Research Institute, Otsuka Pharmaceutical Co., Ltd., 463-10 Kagasuno, Kawauchi-cho, Tokushima 771-0192, Japan.
  • Hatakeyama H; Conference on Experimental Animal Histopathology.
  • Hayashi S; Research Division, Fuji Gotemba Research Laboratories, Chugai Pharmaceutical Co., Ltd., 1-135 Komakado, Gotemba, Shizuoka 412-8513, Japan.
  • Matsumoto I; Conference on Experimental Animal Histopathology.
  • Suzuki S; Ina Research Inc., 2148-188 Nishiminowa, Ina, Nagano 399-4501, Japan.
  • Kotera T; Conference on Experimental Animal Histopathology.
  • Kumagai K; Biological Research Laboratories, Toxicology and Environmental Science Department, Biological Research Laboratories, Nissan Chemical Corporation, 1470 Shiraoka, Shiraoka-shi, Saitama 349-0294, Japan.
  • Ozaki K; Conference on Experimental Animal Histopathology.
J Toxicol Pathol ; 34(2): 161-180, 2021 Apr.
Article em En | MEDLINE | ID: mdl-33976473
ABSTRACT
With the aim of sharing information about the technical aspects of immunohistochemistry (IHC) and facilitating the selection of suitable antibodies for histopathological examination, this technical report describes the results of a questionnaire distributed during the period of 2018 to 2019 among members of the Conference on Experimental Animal Histopathology. Additionally, it describes the immunological properties and supplier details (clone, supplier, catalog number, species reactivity, etc.) as well as the IHC staining conditions (fixing solution, fixing time, embedding, antigen retrieval method, antibody dilution, incubation time, incubation temperature, positive control tissue, blocking condition, secondary antibody information, etc.) for a total of 509 primary antibodies (comprising 220 different types). These survey results were an update on the contents reported by CEAH in 2017.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: J Toxicol Pathol Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: J Toxicol Pathol Ano de publicação: 2021 Tipo de documento: Article