Detection of synovial sepsis in horses using enzymes as biomarkers.
Equine Vet J
; 54(3): 513-522, 2022 May.
Article
em En
| MEDLINE
| ID: mdl-33977535
ABSTRACT
BACKGROUND:
Synovial sepsis is a commonly occurring, potentially career-ending or even life-threatening orthopaedic emergency. Diagnosis of synovial sepsis is currently primarily based on synovial fluid analysis, which often leaves diagnostic ambiguity due to overlap of clinicopathological parameters between septic and aseptic inflammatory synovitis.OBJECTIVES:
To evaluate the reliability of lysozyme (LYS), myeloperoxidase (MPO) and elastase (ELT) as biomarkers for synovial sepsis in horses using a photometric assay to measure increased enzyme activity. STUDYDESIGN:
Prospective, single-blinded, analytical, clinical study.METHODS:
Equine synovial samples were assigned to one of three groups (1) healthy controls (n = 10), (2) aseptic (n = 27) and (3) septic synovitis (n = 30). The enzyme activity assays (LYS, MPO and ELT) were compared with standard synovial fluid parameters and broad-range bacterial 16S rDNA PCR.RESULTS:
LYS and MPO activities were significantly different between septic synovial samples, and both aseptic and control samples (P < .001, LYS confidence interval [CI] 2.25-3.41, resp., 2.21-3.8, MPO CI 0.752-1.6, resp., 0.639-1.81). LYS achieved a 100% sensitivity and 100% specificity in differentiating between septic and aseptic (cut-off value 751.4) or control (cut-off 484.6) samples (P < .001). MPO reached 93.33% sensitivity, 100% specificity for distinguishing septic from control (cut-off value 0.1254) synovial samples and 93.33% sensitivity, 81.48% specificity for discriminating between septic and aseptic (cut-off value 0.1305) synovial samples (P < .001). ELT activity could not be measured in any synovial sample. Both the LYS and the MPO measurements showed a highly significant correlation with PCR (LYS r = .79, MPO r = .69), synovial leukocyte count (LYS r = .752, MPO r = .571), % neutrophils (LYS r = .751, MPO r = 0.663) and each other (r = .744, all P < .001). MAINLIMITATIONS:
Variation in horses' signalment, affected synovial structures and synovial fluid freezing times may have affected the discriminative power of this study.CONCLUSIONS:
Increased MPO and LYS activities allow reliable, rapid diagnosis of synovial sepsis with high sensitivity and specificity.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sinovite
/
Sepse
/
Doenças dos Cavalos
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
Limite:
Animals
Idioma:
En
Revista:
Equine Vet J
Ano de publicação:
2022
Tipo de documento:
Article