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Shifts in the rhizobiome during consecutive in planta enrichment for phosphate-solubilizing bacteria differentially affect maize P status.
De Zutter, Noémie; Ameye, Maarten; Debode, Jane; De Tender, Caroline; Ommeslag, Sarah; Verwaeren, Jan; Vermeir, Pieter; Audenaert, Kris; De Gelder, Leen.
Afiliação
  • De Zutter N; Laboratory of Applied Mycology and Phenomics (LAMP), Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Valentin Vaerwyckweg 1, Ghent, B-9000, Belgium.
  • Ameye M; Laboratory of Environmental Biotechnology, Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Valentin Vaerwyckweg 1, Ghent, B-9000, Belgium.
  • Debode J; Laboratory of Applied Mycology and Phenomics (LAMP), Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Valentin Vaerwyckweg 1, Ghent, B-9000, Belgium.
  • De Tender C; Plant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Burgemeester Van Gansberghelaan 96, Merelbeke, B-9820, Belgium.
  • Ommeslag S; Plant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Burgemeester Van Gansberghelaan 96, Merelbeke, B-9820, Belgium.
  • Verwaeren J; Department of Applied Mathematics, Computer Science and Statistics, Ghent University, Krijgslaan 281 S9, Ghent, B-9000, Belgium.
  • Vermeir P; Plant Sciences Unit, Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Burgemeester Van Gansberghelaan 96, Merelbeke, B-9820, Belgium.
  • Audenaert K; Research Unit Knowledge-based Systems (KERMIT), Department of Data Analysis and Mathematical Modeling, Ghent University, Coupure links 653, Ghent, B-9000, Belgium.
  • De Gelder L; Laboratory of Chemical Analysis (LCA), Faculty of Bioscience Engineering, Ghent University, Valentin Vaerwyckweg 1, Ghent, B-9000, Belgium.
Microb Biotechnol ; 14(4): 1594-1612, 2021 07.
Article em En | MEDLINE | ID: mdl-34021699
Phosphorus (P) is despite its omnipresence in soils often unavailable for plants. Rhizobacteria able to solubilize P are therefore crucial to avoid P deficiency. Selection for phosphate-solubilizing bacteria (PSB) is frequently done in vitro; however, rhizosphere competence is herein overlooked. Therefore, we developed an in planta enrichment concept enabling simultaneous microbial selection for P-solubilization and rhizosphere competence. We used an ecologically relevant combination of iron- and aluminium phosphate to select for PSB in maize (Zea mays L.). In each consecutive enrichment, plant roots were inoculated with rhizobacterial suspensions from plants that had grown in substrate with insoluble P. To assess the plants' P statuses, non-destructive multispectral imaging was used for quantifying anthocyanins, a proxy for maize's P status. After the third consecutive enrichment, plants supplied with insoluble P and inoculated with rhizobacterial suspensions showed a P status similar to plants supplied with soluble P. A parallel metabarcoding approach uncovered that the improved P status in the third enrichment coincided with a shift in the rhizobiome towards bacteria with plant growth-promoting and P-solubilizing capacities. Finally, further consecutive enrichment led to a functional relapse hallmarked by plants with a low P status and a second shift in the rhizobiome at the level of Azospirillaceae and Rhizobiaceae.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microbiologia do Solo / Zea mays Idioma: En Revista: Microb Biotechnol Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microbiologia do Solo / Zea mays Idioma: En Revista: Microb Biotechnol Ano de publicação: 2021 Tipo de documento: Article