Atomic Force Microscopy for Live-Cell and Hydrogel Measurement.

Whitehead, Alexander J; Kirkland, Natalie J; Engler, Adam J

Whitehead, Alexander J; Kirkland, Natalie J; Engler, Adam J.

Afiliação

Whitehead AJ; Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Kirkland NJ; Engler Lab, Sanford Consortium for Regenerative Medicine, La Jolla, CA, USA.
Engler AJ; Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.

Methods Mol Biol ; 2299: 217-226, 2021.

Article em En | MEDLINE | ID: mdl-34028746

ABSTRACT

ABSTRACT

Atomic force microscopy (AFM) has emerged as a popular method for determining the mechanical properties of cells, their components, and biomaterials. Here, we describe AFM setup and application to obtain stiffness measurements from single indentations for hydrogels and myofibroblasts.

Assuntos

Fibroblastos/citologia; Microscopia de Força Atômica/métodos; Miofibroblastos/citologia; Animais; Transdiferenciação Celular; Células Cultivadas; Módulo de Elasticidade; Humanos

Palavras-chave

Atomic force microscopy; Fibroblast; Force-curve; Hydrogels; Live-cell measurement; Stiffness; Young's modulus

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microscopia de Força Atômica / Miofibroblastos / Fibroblastos Limite: Animals / Humans Idioma: En Revista: Methods Mol Biol Ano de publicação: 2021 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google