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Establishment and Characterization of a Novel Human Ocular Adnexal Sebaceous Carcinoma Cell Line.
Rong, Andrew J; Gallo, Ryan A; Zhang, Michelle G; Doddapaneni, Ravi; Griswold, Anthony J; Lee, John Y; Kurtenbach, Stefan; Dubovy, Sander R; Tse, David T; Pelaez, Daniel.
Afiliação
  • Rong AJ; Department of Oculoplastic Surgery, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
  • Gallo RA; Dr. Nasser Ibrahim Al-Rashid Orbital Vision Research Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
  • Zhang MG; Dr. Nasser Ibrahim Al-Rashid Orbital Vision Research Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
  • Doddapaneni R; Ocular Oncology Laboratory, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
  • Griswold AJ; McColl-Lockwood Muscular Dystrophy Laboratory, James G. Cannon Research Center, Atrium Health, Charlotte, NC, USA.
  • Lee JY; John P. Hussman Institute for Human Genomics, University of Miami Miller School of Medicine, Miami, FL, USA.
  • Kurtenbach S; Dr. Nasser Ibrahim Al-Rashid Orbital Vision Research Center, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
  • Dubovy SR; Ocular Oncology Laboratory, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
  • Tse DT; Department of Pathology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
  • Pelaez D; Department of Oculoplastic Surgery, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
Transl Vis Sci Technol ; 10(6): 34, 2021 05 03.
Article em En | MEDLINE | ID: mdl-34043754
ABSTRACT

Purpose:

Sebaceous carcinoma (SC) is a malignant eyelid tumor of the ocular adnexa that is primarily treated via surgical excision. Few therapies exist in advanced cases, and medical therapy is limited because of our incomplete understanding of SC biology. Herein, we describe a technique to culture human ocular adnexal SC for use as an in vitro model.

Methods:

Human ocular adnexal SC tumor cells were isolated from a patient undergoing orbital exenteration surgery and named Bascom Palmer 50 (BP50). They were cultured in Dulbecco's modified Eagle medium/nutrient mixture F-12 supplemented with 10% fetal bovine serum and antibiotics and were maintained at 37°C in humidified 5% CO2. The cells were characterized by immunohistochemistry, exome sequencing, and short tandem repeats analysis. In vitro drug screening against mitomycin-C (MMC) was performed using a cell viability assay.

Results:

BP50 grew past 40 passages with a doubling time of 52.3 hours. Immunocytochemical staining revealed expression of SC-associated markers adipophilin, epithelial membrane antigen, p53, and androgen receptor. Whole exome sequencing showed a significant carryover in somatic mutations between the tumor tissue and corresponding cell line, revealing genetic markers consistent with SC. MMC affected cell viability in a dose-dependent manner.

Conclusions:

BP50 displays characteristics of ocular adnexal SC and therefore may facilitate improved understanding of SC biology and the high throughput assessment of novel therapeutic compounds and new drug combinatorial approaches targeted for this disease. Translational Relevance Drug screening with MMC against these cells shows in vitro evidence to support its continued clinical use in SC.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias das Glândulas Sebáceas / Adenocarcinoma Sebáceo / Neoplasias Oculares / Neoplasias Palpebrais Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Transl Vis Sci Technol Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias das Glândulas Sebáceas / Adenocarcinoma Sebáceo / Neoplasias Oculares / Neoplasias Palpebrais Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Transl Vis Sci Technol Ano de publicação: 2021 Tipo de documento: Article