Modified Blue Native Gel Approach for Analysis of Respiratory Supercomplexes.
Methods Mol Biol
; 2276: 227-234, 2021.
Article
em En
| MEDLINE
| ID: mdl-34060045
ABSTRACT
In mitochondrial oxidative phosphorylation (Ox-Phos), individual electron transport chain complexes are thought to assemble into supramolecular entities termed supercomplexes (SCs). The technique of blue native (BN) gel electrophoresis has emerged as the method of choice for analyzing SCs. However, the process of sample extraction for BN gel analysis is somewhat tedious and introduces the possibility for experimental artifacts. Here we outline a streamlined method that eliminates a centrifugation step and provides a more representative sampling of a population of mitochondria on the final gel. Using this method, we show that SC composition does not appear to change dynamically with altered mitochondrial function.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Mitocondriais
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Complexo de Proteínas da Cadeia de Transporte de Elétrons
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Complexos Multiproteicos
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Eletroforese em Gel de Poliacrilamida Nativa
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Coração
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Mitocôndrias Cardíacas
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Miocárdio
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2021
Tipo de documento:
Article