The critical role of Hedgehog-responsive mesenchymal progenitors in meniscus development and injury repair.
Elife
; 102021 06 04.
Article
em En
| MEDLINE
| ID: mdl-34085927
ABSTRACT
Meniscal tears are associated with a high risk of osteoarthritis but currently have no disease-modifying therapies. Using a Gli1 reporter line, we found that Gli1+ cells contribute to the development of meniscus horns from 2 weeks of age. In adult mice, Gli1+ cells resided at the superficial layer of meniscus and expressed known mesenchymal progenitor markers. In culture, meniscal Gli1+ cells possessed high progenitor activities under the control of Hh signal. Meniscus injury at the anterior horn induced a quick expansion of Gli1-lineage cells. Normally, meniscal tissue healed slowly, leading to cartilage degeneration. Ablation of Gli1+ cells further hindered this repair process. Strikingly, intra-articular injection of Gli1+ meniscal cells or an Hh agonist right after injury accelerated the bridging of the interrupted ends and attenuated signs of osteoarthritis. Taken together, our work identified a novel progenitor population in meniscus and proposes a new treatment for repairing injured meniscus and preventing osteoarthritis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cicatrização
/
Meniscos Tibiais
/
Osteoartrite do Joelho
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Transplante de Células-Tronco Mesenquimais
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Proteínas Hedgehog
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Células-Tronco Mesenquimais
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Lesões do Menisco Tibial
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Proteína GLI1 em Dedos de Zinco
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Elife
Ano de publicação:
2021
Tipo de documento:
Article