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Antibiofilm activity of laser ablation with indocyanine green activated by different power laser parameters compared with photodynamic therapy on root canals infected with Enterococcus faecalis.
Yamamoto, Larissa Yumi; Loureiro, Caroline; Cintra, Luciano Tavares Angelo; Leonardo, Renato de Toledo; Banci, Henrique Augusto; Ribeiro, Ana Paula Fernandes; Sivieri-Araujo, Gustavo; Jacinto, Rogério de Castilho.
Afiliação
  • Yamamoto LY; Department of Preventive and Restorative Dentistry, São Paulo State University (UNESP) School of Dentistry, Araçatuba, SP, Brazil. Electronic address: lariyamamoto.lyy@gmail.com.
  • Loureiro C; Department of Preventive and Restorative Dentistry, São Paulo State University (UNESP) School of Dentistry, Araçatuba, SP, Brazil. Electronic address: caroline.loureiro@unesp.br.
  • Cintra LTA; Department of Preventive and Restorative Dentistry, São Paulo State University (UNESP) School of Dentistry, Araçatuba, SP, Brazil. Electronic address: luciano.cintra@unesp.br.
  • Leonardo RT; Department of Restorative Dentistry, São Paulo State University (UNESP) School of Dentistry, Araraquara, SP, Brazil. Electronic address: renato.leonardi@unesp.br.
  • Banci HA; Department of Preventive and Restorative Dentistry, São Paulo State University (UNESP) School of Dentistry, Araçatuba, SP, Brazil. Electronic address: henrique.banci@unesp.br.
  • Ribeiro APF; Department of Preventive and Restorative Dentistry, São Paulo State University (UNESP) School of Dentistry, Araçatuba, SP, Brazil. Electronic address: apf.ribeiro@unesp.br.
  • Sivieri-Araujo G; Department of Preventive and Restorative Dentistry, São Paulo State University (UNESP) School of Dentistry, Araçatuba, SP, Brazil. Electronic address: gustavo.sivieri@unesp.br.
  • Jacinto RC; Department of Preventive and Restorative Dentistry, São Paulo State University (UNESP) School of Dentistry, Araçatuba, SP, Brazil. Electronic address: rogerio.castilho@unesp.br.
Photodiagnosis Photodyn Ther ; 35: 102377, 2021 Sep.
Article em En | MEDLINE | ID: mdl-34087469
BACKGROUND: Antimicrobial photodynamic therapy (aPDT) is used as an adjunct to endodontic treatment to enhance microbial reduction in the root canal system. However, studies evaluating the impact of laser ablation with Indocyanine Green (ICG) are scarce. Thus, this in vitro study aimed to evaluate the efficiency of laser ablation with ICG using different laser parameters compared with aPDT using photosensitizer methylene blue (MB) and curcumin (CUR) on the reduction of E. faecalis biofilms on root canals. METHODS: Forty-nine human premolars were used after biomechanical instrumentation for standardization. The root canals were contaminated with E. faecalis for 10 days to form biofilms, and divided into 7 groups (n = 7): 0.01% MB activated by red laser - MB+RL; 0.05% CUR activated by blue LED - CUR+BL; 0.05% ICG activated by infrared diode laser (2.5 W power, 30 ms interval, and 30 ms duration) - ICG+DL 2.5/30/30; 0.05% ICG activated by infrared diode laser (2.5 W power, 300 ms interval, and 100 ms duration) - ICG+DL 2.5/300/100; 0.05% ICG activated by infrared diode laser (3 W power, 300 ms interval, and 100 ms duration) - ICG+DL 3/300/100; sterile saline solution (negative control) - NC; and 2.5% NaOCl (positive control) - PC. Root canal sampling was performed prior to and immediately after the different treatment protocols. Data were submitted to One- and Two-Way ANOVA, followed by Student-Newman-Keuls test or Fisher LSD's test (α = 0.05). RESULTS: All aPDT protocols promoted significant CFU reductions compared with the NC; the highest CFU reduction was observed for PC (p < 0.05). Among the protocols the highest CFU reduction was promoted by laser ablation with ICG+DL 3/300/100 (p < 0.05) except compared with aPDT using CUR+BL (p > 0.05). CONCLUSIONS: Laser ablation protocol using ICG+DL proved to be efficient in reducing E. faecalis biofilms, especially when activated at 3/300/100 configuration.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Terapia a Laser Tipo de estudo: Guideline Limite: Humans Idioma: En Revista: Photodiagnosis Photodyn Ther Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Terapia a Laser Tipo de estudo: Guideline Limite: Humans Idioma: En Revista: Photodiagnosis Photodyn Ther Ano de publicação: 2021 Tipo de documento: Article