Rapid multiplex PCR assays in patients with respiratory viral infections: is semi-quantitative data useful? A pilot study.
Braz J Microbiol
; 52(3): 1173-1179, 2021 Sep.
Article
em En
| MEDLINE
| ID: mdl-34105111
ABSTRACT
Viral respiratory rapid multiplex PCR assays FilmArray® (FA) and ePlex® (eP) provide qualitative results which may not reflect clinical relevance. In a pilot study, we report retrospectively whether the semi-quantitative PCR assay R-GENE® would have facilitated clinical interpretation. Forty-four patients were hospitalized for various respiratory manifestations; all of them have benefited from a respiratory sample during acute symptoms. Among the 44 patients, FA detected 23 positive samples including 31 viruses, 26 of them gave high or moderate R-GENE® scores (cycle threshold < 35), and all but one were consistent with clinical history. Semi-quantitative scores would have allowed for critical interpretation of the results; those are a key additional element for an optimal exploitation of the rapid multiplex PCR assays power.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções Respiratórias
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Vírus
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Viroses
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Reação em Cadeia da Polimerase Multiplex
Tipo de estudo:
Diagnostic_studies
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Observational_studies
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Qualitative_research
Limite:
Humans
Idioma:
En
Revista:
Braz J Microbiol
Ano de publicação:
2021
Tipo de documento:
Article