Single-unit Recording in Awake Behaving Non-human Primates.
Bio Protoc
; 11(8): e3987, 2021 Apr 20.
Article
em En
| MEDLINE
| ID: mdl-34124290
ABSTRACT
Non-human primates (NHPs) have been widely used as a species model in studies to understand higher brain functions in health and disease. These studies employ specifically designed behavioral tasks in which animal behavior is well-controlled, and record neuronal activity at high spatial and temporal resolutions while animals are performing the tasks. Here, we present a detailed procedure to conduct single-unit recording, which fulfils high spatial and temporal resolutions while macaque monkeys (i.e., widely used NHPs) perform behavioral tasks in a well-controlled manner. This procedure was used in our previous study to investigate the dynamics of neuronal activity during economic decision-making by the monkeys. Monkeys' behavior was quantitated by eye position tracking and button press/release detection. By inserting a microelectrode into the brain, with a grid system in reference to magnetic resonance imaging, we precisely recorded the brain regions. Our experimental system permits rigorous investigation of the link between neuronal activity and behavior.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Bio Protoc
Ano de publicação:
2021
Tipo de documento:
Article