Bone Marrow Mononuclear Cells Transplantation and Training Increased Transplantation of Energy Source Transporters in Chronic Stroke.
J Stroke Cerebrovasc Dis
; 30(8): 105932, 2021 Aug.
Article
em En
| MEDLINE
| ID: mdl-34148020
ABSTRACT
OBJECTIVES:
Bone marrow mononuclear cells (BM-MNC) show a significant therapeutic effect in combination with training even in the chronic phase of stroke. However, the mechanism of this combination therapy has not been investigated. Here, we examined its effects on brain metabolism in chronic stroke mice. MATERIALS ANDMETHODS:
BM-MNC (1x105 cells in 100 µL of phosphate-buffered saline) were intravenously transplanted at 4 weeks (chronic stage) after the middle cerebral artery occlusion. At 3 h and 10 weeks after the administration of BM-MNC, we evaluated transcription changes of the metabolism-related genes, hypoxia inducible factor 1-α (Hif-1α), prolyl hydroxylase 3 (Phd3), pyruvate dehydrogenase kinase 1 (Pdk1), Na+/K+-ATPase (Atp1α1â3), connexins, glucose transporters, and monocarboxylate transporters, in the brain during chronic phase of stroke using quantitative polymerase chain reaction.RESULTS:
The results showed transcriptional activation of the metabolism-related genes in the contralateral cortex at 3 h after BM-MNC transplantation. Behavioral tests were performed after cell therapy, and the brain metabolism of mice with improved motor function was examined at 10 weeks after cell therapy. The therapeutic efficacy of the combination therapy with BM-MNC transplantation and training was evident in the form of transcriptional activation of ipsilateral anterior cerebral artery (ACA) cortex.CONCLUSIONS:
BM-MNC transplantation combined with training for chronic stroke activated gene expression in both the ipsilateral and the contralateral side.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Condicionamento Físico Animal
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Encéfalo
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Células da Medula Óssea
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Transplante de Medula Óssea
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Infarto da Artéria Cerebral Média
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Metabolismo Energético
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Stroke Cerebrovasc Dis
Ano de publicação:
2021
Tipo de documento:
Article