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Label-free biosensor of phagocytosis for diagnosing bacterial infections.
Liao, Junchen; Ren, Jifeng; Wei, Huang; Lam, Raymond H W; Chua, Song Lin; Khoo, Bee Luan.
Afiliação
  • Liao J; Department of Biomedical Engineering, City University of Hong Kong, Hong Kong SAR, China.
  • Ren J; Department of Biomedical Engineering, City University of Hong Kong, Hong Kong SAR, China; School of Biomedical Engineering, Capital Medical University, Beijing, 100069, China.
  • Wei H; Department of Biomedical Engineering, City University of Hong Kong, Hong Kong SAR, China.
  • Lam RHW; Department of Biomedical Engineering, City University of Hong Kong, Hong Kong SAR, China; City University of Hong Kong Shenzhen Research Institute, Shenzhen, 518057, China; Centre for Robotics and Automation, City University of Hong Kong, Hong Kong SAR, China.
  • Chua SL; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong SAR, 999077, China; State Key Laboratory of Chemical Biology and Drug Discovery, The Hong Kong Polytechnic University, Hong Kong SAR, 999077, China; Shenzhen Key Laboratory of Food Biological Safet
  • Khoo BL; Department of Biomedical Engineering, City University of Hong Kong, Hong Kong SAR, China. Electronic address: blkhoo@cityu.edu.hk.
Biosens Bioelectron ; 191: 113412, 2021 Nov 01.
Article em En | MEDLINE | ID: mdl-34153636
Phagocytic cells recognize and phagocytose invading microbes for destruction. However, bacterial pathogens can remain hidden at low levels from conventional detection or replicate intracellularly after being phagocytosed by immune cells. Current phagocytosis-detection approaches involve flow cytometry or microscopic search for rare bacteria-internalized phagocytes among large populations of uninfected cells, which poses significant challenges in research and clinical settings. Hence it is imperative to develop a rapid, non-disruptive, and label-free phagocytosis detection approach. Using deformability assays and microscopic imaging, we have demonstrated for the first time that the presence of intracellular bacteria in phagocytic blood cells led to aberrant physical properties. Specifically, human monocytes with internalized bacteria of various species were stiffer and larger compared with uninfected monocytes. Taking advantage of these physical differences, a novel microfluidics-based biosensor platform was developed to passively sort, concentrate and quantify rare monocytes with internalized pathogens (MIP) from uninfected monocyte populations for phagocytosis detection. The clinical utility of the MIP platform was demonstrated by enriching and detecting bacteria-internalized monocytes from spiked human blood samples within 1.5 h. Patient-derived clinical isolates were used to validate the utility of the MIP platform further. This proof-of-concept presents a phagocytosis detection platform that could be used to rapidly diagnose microbial infections, especially in bloodstream infections (BSIs), thereby improving the clinical outcomes for point-of-care management.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Bacterianas / Técnicas Biossensoriais Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Biosens Bioelectron Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Bacterianas / Técnicas Biossensoriais Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Biosens Bioelectron Ano de publicação: 2021 Tipo de documento: Article