Optimization of a human milk-directed quantitative sIgA ELISA method substantiated by mass spectrometry.

Dingess, Kelly A; van Dam, Pauline; Zhu, Jing; Mank, Marko; Knipping, Karen; Heck, Albert J R; Stahl, Bernd

Dingess, Kelly A; van Dam, Pauline; Zhu, Jing; Mank, Marko; Knipping, Karen; Heck, Albert J R; Stahl, Bernd.

Afiliação

Dingess KA; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, Utrecht, 3584 CH, The Netherlands.
van Dam P; Netherlands Proteomics Center, Padualaan 8, Utrecht, 3584 CH, The Netherlands.
Zhu J; Danone Nutricia Research, Uppsalalaan 12, Utrecht, 3584 CT, The Netherlands.
Mank M; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Padualaan 8, Utrecht, 3584 CH, The Netherlands.
Knipping K; Netherlands Proteomics Center, Padualaan 8, Utrecht, 3584 CH, The Netherlands.
Heck AJR; Beijing Institute of Nutritional Resources, Beijing, 100069, China.
Stahl B; Danone Nutricia Research, Uppsalalaan 12, Utrecht, 3584 CT, The Netherlands.

Anal Bioanal Chem ; 413(20): 5037-5049, 2021 Aug.

Article em En | MEDLINE | ID: mdl-34169348

Assuntos

Ensaio de Imunoadsorção Enzimática/métodos; Imunoglobulina A Secretora/química; Espectrometria de Massas/métodos; Leite Humano/química; Feminino; Humanos; Imunoglobulina G/química; Lactação; Reprodutibilidade dos Testes

Palavras-chave

ELISA; Human milk; Mass spectrometry; Proteomics; sIgA

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Imunoglobulina A Secretora / Ensaio de Imunoadsorção Enzimática / Leite Humano Limite: Female / Humans Idioma: En Revista: Anal Bioanal Chem Ano de publicação: 2021 Tipo de documento: Article

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