Bacteria-fungal Confrontation and Fungal Growth Prevention Assay.
Bio Protoc
; 8(2): e2694, 2018 Jan 20.
Article
em En
| MEDLINE
| ID: mdl-34179243
ABSTRACT
There are some bacteria which can grow and multiply at the cost of living fungal biomass. They can potentially utilize fungi as a source of nutrients to forage over them. Such phenomenon is known as bacterial mycophagy, however, its mechanistic insights need to be explored to identify the molecules involved in mycophagy for potential utilization in controlling various fungal diseases. Recently we have demonstrated that a rice-associated bacteria Burkholderia gladioli strain NGJ1 exhibits mycophagous ability on several fungi, including Rhizoctonia solani, the necrotrophic fungal pathogen causing sheath blight disease in rice. We hereby describe our validated and efficient methods used to study B. gladioli strain NGJ1-R. solani interactions. These methodologies would be useful for designing assays to study the confrontation between bacteria and fungi which in turn enable discovery of novel antifungal molecules from such bacteria.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Bio Protoc
Ano de publicação:
2018
Tipo de documento:
Article