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Sticholysin II shows similar immunostimulatory properties to LLO stimulating dendritic cells and MHC-I restricted T cell responses of heterologous antigen.
Del Valle, A; Acosta-Rivero, N; Laborde, R J; Cruz-Leal, Y; Cabezas, S; Luzardo, M C; Alvarez, C; Labrada, M; Rodríguez, A; Rodríguez, G L; Raymond, J; Nogueira, C V; Grubaugh, D; Fernández, L E; Higgins, D; Lanio, M E.
Afiliação
  • Del Valle A; Center for Protein Studies, Faculty of Biology, Havana University (UH) and Lab UH-CIM, Cuba.
  • Acosta-Rivero N; Center for Protein Studies, Faculty of Biology, Havana University (UH) and Lab UH-CIM, Cuba. Electronic address: nelson.acosta@fbio.uh.cu.
  • Laborde RJ; Center for Protein Studies, Faculty of Biology, Havana University (UH) and Lab UH-CIM, Cuba.
  • Cruz-Leal Y; Center for Protein Studies, Faculty of Biology, Havana University (UH) and Lab UH-CIM, Cuba.
  • Cabezas S; Center for Protein Studies, Faculty of Biology, Havana University (UH) and Lab UH-CIM, Cuba.
  • Luzardo MC; Center for Protein Studies, Faculty of Biology, Havana University (UH) and Lab UH-CIM, Cuba.
  • Alvarez C; Center for Protein Studies, Faculty of Biology, Havana University (UH) and Lab UH-CIM, Cuba.
  • Labrada M; Center of Molecular Immunology (CIM), Playa, La Habana, Cuba.
  • Rodríguez A; Center of Molecular Immunology (CIM), Playa, La Habana, Cuba.
  • Rodríguez GL; Center of Molecular Immunology (CIM), Playa, La Habana, Cuba.
  • Raymond J; Center of Molecular Immunology (CIM), Playa, La Habana, Cuba.
  • Nogueira CV; Harvard Medical School, Harvard University, USA.
  • Grubaugh D; Harvard Medical School, Harvard University, USA.
  • Fernández LE; Center of Molecular Immunology (CIM), Playa, La Habana, Cuba.
  • Higgins D; Harvard Medical School, Harvard University, USA.
  • Lanio ME; Center for Protein Studies, Faculty of Biology, Havana University (UH) and Lab UH-CIM, Cuba. Electronic address: mlanio@fbio.uh.cu.
Toxicon ; 200: 38-47, 2021 Sep.
Article em En | MEDLINE | ID: mdl-34237340
ABSTRACT
Induction of CD8+ T cell responses against tumor cells and intracellular pathogens is an important goal of modern vaccinology. One approach of translational interest is the use of liposomes encapsulating pore-forming proteins (PFPs), such as Listeriolysin O (LLO), which has shown efficacy at priming strong and sustained CD8+ T cell responses. Recently, we have demonstrated that Sticholysin II (StII), a PFP from the sea anemone Stichodactyla helianthus, co-encapsulated into liposomes with ovalbumin (OVA) was able to stimulate, antigen presenting cells, antigen-specific CD8+ T cells and anti-tumor activity in mice. In the present study, we aimed to compare StII and LLO in terms of their abilities to stimulate dendritic cells and to induce major histocompatibility complex (MHC) class I restricted T cell responses against OVA. Interestingly, StII exhibited similar abilities to LLO in vitro of inducing dendritic cells maturation, as measured by increased expression of CD40, CD80, CD86 and MHC-class II molecules, and of stimulating OVA cross-presentation to a CD8+ T cell line. Remarkably, using an ex vivo Enzyme-Linked ImmunoSpot Assay (ELISPOT) to monitor gamma interferon (INF-γ) producing effector memory CD8+ T cells, liposomal formulations containing either StII or LLO induced comparable frequencies of OVA-specific INF-γ producing CD8+ T cells in mice that were sustained in time. However, StII-containing liposomes stimulated antigen-specific memory CD8+ T cells with a higher potential to secrete IFN-γ than liposomes encapsulating LLO. This StII immunostimulatory property further supports its use for the rational design of T cell vaccines against cancers and intracellular pathogens. In summary, this study indicates that StII has immunostimulatory properties similar to LLO, despite being evolutionarily distant PFPs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos T Citotóxicos / Linfócitos T CD8-Positivos Limite: Animals Idioma: En Revista: Toxicon Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos T Citotóxicos / Linfócitos T CD8-Positivos Limite: Animals Idioma: En Revista: Toxicon Ano de publicação: 2021 Tipo de documento: Article