Effect of beta-decay of radionuclides incorporated into influenza virus RNA and proteins on the infectivity of the virus and antigenicity of its nucleoprotein.
Arch Virol
; 97(3-4): 325-32, 1987.
Article
em En
| MEDLINE
| ID: mdl-3426399
ABSTRACT
The effect of beta-decay of radionuclides incorporated into influenza virus on the properties of the two closely adjacent structures--RNA and nucleoprotein (NP)--was studied. The long-term storage of 3H-uridine labelled influenza virus was shown to lead to the loss of infectivity. This effect may be explained by lethal intra-molecular modifications of viral RNA, caused by beta-decay of 3H incorporated into the molecule. There was an accompanying decrease of monoclonal antibody (MAB) binding activity, this also being a plausible result of beta-decay. The different rates of inactivation of MAB binding activity of different epitopes of NP of the 3H-labelled virus shown in our studies suggest that there are different types of structural organization or different location of these epitopes in the NP. The effect of 3H-decay on the intracellular RNA of reproducing virus lead to a decrease in virus yield; this may be due to radiation- and transmutation-induced damage of messenger and progeny RNA populations synthesized during the infection. The storage of influenza virus labelled with 14C-aminoacids lead to a decrease in MAB binding activity of the NP that was unaccompanied by a decrease in infectivity. Furthermore, 14C-decay in proteins of reproducing virus had no adverse effect.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Influenza A
/
Proteínas Virais
/
RNA Viral
/
Antígenos Virais
/
Nucleoproteínas
Limite:
Animals
Idioma:
En
Revista:
Arch Virol
Ano de publicação:
1987
Tipo de documento:
Article