Antagonist targeting miR106b5p attenuates acute renal injury by regulating renal function, apoptosis and autophagy via the upregulation of TCF4.
Int J Mol Med
; 48(3)2021 Sep.
Article
em En
| MEDLINE
| ID: mdl-34278441
ABSTRACT
Acute renal injury (ARI) is a lifethreatening condition and a main contributor to endstage renal disease, which is mainly caused by ischemiareperfusion (I/R). miR106b5p is a kidney functionrelated miRNA; however, whether miR106b5p regulates the progression of ARI remains unclear. The present study thus aimed to examine the effects of miR106b5p antagonist on the regulation of ARI progression. It was found that miR106b5p expression was upregulated in the renal tissue of rats with I/Rinduced ARI and in NRK52E rat renal proximal tubular epithelial cells subjected to hypoxiareoxygenation (H/R). In vitro, H/R induction suppressed the proliferation, and promoted the apoptosis and autophagy of NRK52E cells, whereas miR106b5p antagonist (inhibition of miR106b5p) promoted the proliferation, and attenuated the apoptosis and autophagy of NRK52E cells under the H/R condition. Dual luciferase reporter gene assay validated that transcription factor 4 (TCF4) was a target of miR106b5p. It was further found that TCF4 overexpression promoted the proliferation, and inhibited the apoptosis and autophagy of NRK52E cells subjected to H/R. Moreover, the effects of miR106b5p antagonist on NRK52E cell proliferation, apoptosis and autophagy were mediated through the regulation of TCF4. In vivo, miR106b5p antagonist reduced the severity of renal injury, decreased cell proliferation in renal tissues and lowered the serum creatinine (Scr) and blood urea nitrogen (BUN) levels in the blood samples from rats with I/Rinduced ARI. On the whole, the findings presented herein demonstrate that miR106b5p antagonist attenuates ARI by promoting the proliferation, and suppressing the apoptosis and autophagy of renal cells via upregulating TCF4.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
6_ODS3_enfermedades_notrasmisibles
Base de dados:
MEDLINE
Assunto principal:
Apoptose
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MicroRNAs
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Injúria Renal Aguda
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Antagomirs
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Fator de Transcrição 4
Limite:
Animals
Idioma:
En
Revista:
Int J Mol Med
Ano de publicação:
2021
Tipo de documento:
Article