HLA-B*27 typing using a triplex real time PCR in routine laboratory.

Viken, Marte K; Pedersen, Alice L; Andersen, Menaka; Jensen, Tore; Lie, Benedicte A; Boulland, Line M L

Viken, Marte K; Pedersen, Alice L; Andersen, Menaka; Jensen, Tore; Lie, Benedicte A; Boulland, Line M L.

Afiliação

Viken MK; Department of Immunology, Oslo University Hospital and University of Oslo, Oslo, Norway.
Pedersen AL; Department of Immunology, Oslo University Hospital and University of Oslo, Oslo, Norway.
Andersen M; Department of Immunology, Oslo University Hospital and University of Oslo, Oslo, Norway.
Jensen T; Department of Immunology, Oslo University Hospital and University of Oslo, Oslo, Norway.
Lie BA; Department of Immunology, Oslo University Hospital and University of Oslo, Oslo, Norway.
Boulland LML; Deptartment of medical genetics, Oslo University Hospital and University of Oslo, Oslo, Norway.

HLA ; 98(4): 366-369, 2021 10.

Article em En | MEDLINE | ID: mdl-34342381

ABSTRACT

ABSTRACT

We have established an in-house HLA-B*27 multiplex typing assay by using a combination of previously published, newly designed and commercial primers and probes for use with real-time PCR instruments. Hence, facilitating quick and large-scale HLA-B*27 typing.

Assuntos

Antígenos HLA-B; Laboratórios; Alelos; Antígenos HLA-B/genética; Teste de Histocompatibilidade; Humanos; Reação em Cadeia da Polimerase em Tempo Real

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antígenos HLA-B / Laboratórios Limite: Humans Idioma: En Revista: HLA Ano de publicação: 2021 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google