A fast, nondestructive method for the detection of disease-related lesions and wounded leaves.
Biotechniques
; 71(2): 425-430, 2021 08.
Article
em En
| MEDLINE
| ID: mdl-34374299
ABSTRACT
Trypan blue staining is a classic way of visualizing leaf disease and wound responses in plants, but it involves working with toxic chemicals and is time-consuming (2-3 days). Here, the investigators established near-infrared scanning with standard lab equipment as a fast and nondestructive method for the analysis of leaf injuries compared with trypan blue staining. Pathogen-inoculated and wounded leaves from potato, tomato, spinach, strawberry, and arabidopsis plants were used for proof of concept. The results showed that this newly developed protocol with near-infrared scanning gave the same results as trypan blue staining. Furthermore, a macro in FIJI was made to quantify the leaf damage. The new protocol was time-efficient, nondestructive, chemical-free and may be used for high-throughput studies.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Azul Tripano
/
Arabidopsis
/
Folhas de Planta
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
2021
Tipo de documento:
Article