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Spatiotemporal extracellular matrix modeling for in situ cell niche studies.
Olesen, Kim; Rodin, Sergey; Mak, Wing Cheung; Felldin, Ulrika; Österholm, Cecilia; Tilevik, Andreas; Grinnemo, Karl-Henrik.
Afiliação
  • Olesen K; Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.
  • Rodin S; School of Bioscience, University of Skövde, Skövde, Sweden.
  • Mak WC; Polymer Chemistry, Department of Chemistry - Ångström Laboratory, Uppsala University, Uppsala, Sweden.
  • Felldin U; Department of Surgical Sciences, Division of Cardiothoracic Surgery and Anaesthesiology, Uppsala University, Akademiska University Hospital, Uppsala, Sweden.
  • Österholm C; Biosensors and Bioelectronics Centre, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden.
  • Tilevik A; Department of Surgical Sciences, Division of Cardiothoracic Surgery and Anaesthesiology, Uppsala University, Akademiska University Hospital, Uppsala, Sweden.
  • Grinnemo KH; Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.
Stem Cells ; 39(12): 1751-1765, 2021 12.
Article em En | MEDLINE | ID: mdl-34418223
ABSTRACT
Extracellular matrix (ECM) components govern a range of cell functions, such as migration, proliferation, maintenance of stemness, and differentiation. Cell niches that harbor stem-/progenitor cells, with matching ECM, have been shown in a range of organs, although their presence in the heart is still under debate. Determining niches depends on a range of in vitro and in vivo models and techniques, where animal models are powerful tools for studying cell-ECM dynamics; however, they are costly and time-consuming to use. In vitro models based on recombinant ECM proteins lack the complexity of the in vivo ECM. To address these issues, we present the spatiotemporal extracellular matrix model for studies of cell-ECM dynamics, such as cell niches. This model combines gentle decellularization and sectioning of cardiac tissue, allowing retention of a complex ECM, with recellularization and subsequent image processing using image stitching, segmentation, automatic binning, and generation of cluster maps. We have thereby developed an in situ representation of the cardiac ECM that is useful for assessment of repopulation dynamics and to study the effect of local ECM composition on phenotype preservation of reseeded mesenchymal progenitor cells. This model provides a platform for studies of organ-specific cell-ECM dynamics and identification of potential cell niches.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Matriz Extracelular / Células-Tronco Mesenquimais Limite: Animals Idioma: En Revista: Stem Cells Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Matriz Extracelular / Células-Tronco Mesenquimais Limite: Animals Idioma: En Revista: Stem Cells Ano de publicação: 2021 Tipo de documento: Article