Deoxynivalenol and Zearalenone: Different Mycotoxins with Different Toxic Effects in the Sertoli Cells of Equus asinus.
Cells
; 10(8)2021 07 27.
Article
em En
| MEDLINE
| ID: mdl-34440667
ABSTRACT
(1) Background:
Deoxynivalenol (DON) and zearalenone (ZEA) are type B trichothecene mycotoxins that exert serious toxic effects on the reproduction of domestic animals. However, there is little information about the toxicity of mycotoxins on testis development in Equus asinus. This study investigated the biological effects of DON and ZEA exposure on Sertoli cells (SCs) of Equus asinus; (2)Methods:
We administered 10 µM and 30 µM DON and ZEA to cells cultured in vitro; (3)Results:
The results showed that 10 µM DON exposure remarkably changed pyroptosis-associated genes and that 30 µM ZEA exposure changed inflammation-associated genes in SCs. The mRNA expression of cancer-promoting genes was remarkably upregulated in the cells exposed to DON or 30 µM ZEA; in particular, DON and ZEA remarkably disturbed the expression of androgen and oestrogen secretion-related genes. Furthermore, quantitative RT-PCR, Western blot, and immunofluorescence analyses verified the different expression patterns of related genes in DON- and ZEA-exposed SCs; (4)Conclusions:
Collectively, these results illustrated the impact of exposure to different toxins and concrete toxicity on the mRNA expression of SCs from Equus asinus in vitro.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células de Sertoli
/
Tricotecenos
/
Zearalenona
/
Transcriptoma
Limite:
Animals
Idioma:
En
Revista:
Cells
Ano de publicação:
2021
Tipo de documento:
Article