A surface-based calibration approach to enable dynamic and accurate quantification of colorimetric assay systems.
Anal Methods
; 13(37): 4290-4297, 2021 09 30.
Article
em En
| MEDLINE
| ID: mdl-34473147
ABSTRACT
Colorimetry is widely used in assay systems for its low-cost, ease-of-use, rapidity, moderate storage requirements and intuitively visible effects. However, the application is limited due to its relatively low sensitivity. Conventional colorimetric calibration methods often use a fixed incubation time that can limit the detection range, system robustness and sensitivity. In this paper, we used color saturation to measure the accumulation of product (correlation coefficient R2 = 0.9872), and we created a novel "calibration mesh" method based on an expanded sigmoid function to enhance sensitivity. The novel calibration mesh method can be adapted for a wide variety of assay systems to improve robustness and detection range, and provide a dynamic and faster output.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bioensaio
/
Colorimetria
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Anal Methods
Ano de publicação:
2021
Tipo de documento:
Article