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Development and application of a SARS-CoV-2 colorimetric biosensor based on the peroxidase-mimic activity of γ-Fe2O3 nanoparticles.
Büyüksünetçi, Yudum Tepeli; Çitil, Burak Ekrem; Tapan, Utku; Anik, Ülkü.
Afiliação
  • Büyüksünetçi YT; Faculty of Science, Chemistry Department, Mugla Sitki Kocman University, 48000, Kotekli, Mugla, Turkey.
  • Çitil BE; Faculty of Medicine, Department of Medical Microbiology, Mugla Sitki Kocman University, Kotekli, Mugla, Turkey, 4800.
  • Tapan U; Faculty of Medicine, Department of Chest Diseases, Mugla Sitki Kocman University, Kotekli, Mugla, Turkey, 4800.
  • Anik Ü; Faculty of Science, Chemistry Department, Mugla Sitki Kocman University, 48000, Kotekli, Mugla, Turkey. ulkuanik@mu.edu.tr.
Mikrochim Acta ; 188(10): 335, 2021 Sep 09.
Article em En | MEDLINE | ID: mdl-34505191
A practical colorimetric assay was developed for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For this purpose, magnetic γ Fe2O3 nanoparticles were synthesized and used as a peroxidase-like mimic activity molecule. In the presence of γ Fe2O3 nanoparticles, the color change of H2O2 included 3,3',5,5'-tetramethylbenzidine was monitored at the wavelength of 654 nm when spike protein interacted with angiotensin-converting enzyme 2 receptor. This oxidation-reduction reaction was examined both spectroscopically and by using electrochemical techniques. The experimental parameters were optimized and the analytical characteristics investigated. The developed assay was applied to real SARS-CoV-2 samples, and very good results that were in accordance with the real time polymerase chain reaction were obtained.
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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 4_TD Base de dados: MEDLINE Assunto principal: Colorimetria / Glicoproteína da Espícula de Coronavírus / Nanopartículas Magnéticas de Óxido de Ferro / Teste para COVID-19 / SARS-CoV-2 / COVID-19 Limite: Humans Idioma: En Revista: Mikrochim Acta Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 4_TD Base de dados: MEDLINE Assunto principal: Colorimetria / Glicoproteína da Espícula de Coronavírus / Nanopartículas Magnéticas de Óxido de Ferro / Teste para COVID-19 / SARS-CoV-2 / COVID-19 Limite: Humans Idioma: En Revista: Mikrochim Acta Ano de publicação: 2021 Tipo de documento: Article