Isolation and Detection of the Chlorophyll Catabolite Hydroxylating Activity from Capsicum annuum Chromoplasts.
Bio Protoc
; 7(18): e2561, 2017 Sep 20.
Article
em En
| MEDLINE
| ID: mdl-34541205
ABSTRACT
Hydroxylation of chlorophyll catabolites at the so-called C32 position ( Hauenstein et al., 2016 ) is commonly found in all plant species analyzed to date. Here we describe an in vitro hydroxylation assay using Capsicum annuum chromoplast membranes as a source of the hydroxylating activity, which converts the substrate epi-pFCC (epi-primary Fluorescent Chlorophyll Catabolite) ( Mühlecker et al., 2000 ) to epi-pFCC-OH.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Bio Protoc
Ano de publicação:
2017
Tipo de documento:
Article