In vivo, in vitro and in silico: an open space for the development of microbe-based applications of synthetic biology.
Microb Biotechnol
; 15(1): 42-64, 2022 01.
Article
em En
| MEDLINE
| ID: mdl-34570957
ABSTRACT
Living systems are studied using three complementary approaches living cells, cell-free systems and computer-mediated modelling. Progresses in understanding, allowing researchers to create novel chassis and industrial processes rest on a cycle that combines in vivo, in vitro and in silico studies. This design-build-test-learn iteration loop cycle between experiments and analyses combines together physiology, genetics, biochemistry and bioinformatics in a way that keeps going forward. Because computer-aided approaches are not directly constrained by the material nature of the entities of interest, we illustrate here how this virtuous cycle allows researchers to explore chemistry which is foreign to that present in extant life, from whole chassis to novel metabolic cycles. Particular emphasis is placed on the importance of evolution.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biologia Computacional
/
Biologia Sintética
Idioma:
En
Revista:
Microb Biotechnol
Ano de publicação:
2022
Tipo de documento:
Article