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Alternative strategies for the synthesis of [11C]ER176 for PET imaging of neuroinflammation.
Mixdorf, Jason C; Murali, Dhanabalan; Xin, Yangchun; DiFilippo, Alexandra H; Aluicio-Sarduy, Eduardo; Barnhart, Todd E; Engle, Jonathan W; Ellison, Paul A; Christian, Bradley T.
Afiliação
  • Mixdorf JC; Departments of Medical Physics, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI, USA; Waisman Laboratory for Brain Imaging and Behavior, University of Wisconsin-Madison, Madison, WI, USA. Electronic address: jmixdorf@wisc.edu.
  • Murali D; Waisman Laboratory for Brain Imaging and Behavior, University of Wisconsin-Madison, Madison, WI, USA.
  • Xin Y; Waisman Laboratory for Brain Imaging and Behavior, University of Wisconsin-Madison, Madison, WI, USA.
  • DiFilippo AH; Departments of Medical Physics, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI, USA; Waisman Laboratory for Brain Imaging and Behavior, University of Wisconsin-Madison, Madison, WI, USA.
  • Aluicio-Sarduy E; Departments of Medical Physics, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI, USA.
  • Barnhart TE; Departments of Medical Physics, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI, USA.
  • Engle JW; Departments of Medical Physics, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI, USA; Radiology, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI, USA.
  • Ellison PA; Departments of Medical Physics, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI, USA.
  • Christian BT; Departments of Medical Physics, University of Wisconsin-Madison School of Medicine and Public Health, Madison, WI, USA; Waisman Laboratory for Brain Imaging and Behavior, University of Wisconsin-Madison, Madison, WI, USA.
Appl Radiat Isot ; 178: 109954, 2021 Dec.
Article em En | MEDLINE | ID: mdl-34607293
ABSTRACT
[11C]ER176 is a next generation PET radioligand for imaging 18 kDa translocator protein, a biomarker for neuroinflammation. The goal of this work was to investigate alternative strategies for the radiochemical synthesis, purification, and formulation of [11C]ER176. An optimized tri-solvent high-performance liquid chromatography (HPLC) protocol is described to separate the hydro-de-chlorinated byproduct from [11C]ER176. A newly implemented solid phase extraction work-up efficiently removed HPLC solvent while maintaining chemical purity and overall radiochemical yield and purity. This new HPLC purification and final formulation was completed within 40 min, providing 2.7 ± 0.5 GBq of [11C]ER176 at end of synthesis with 1400 ± 300 GBq/µmol molar activity while meeting all specifications for radiopharmaceutical quality control tests for human research use.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Radioisótopos de Carbono / Compostos Radiofarmacêuticos / Tomografia por Emissão de Pósitrons / Doenças Neuroinflamatórias Limite: Humans Idioma: En Revista: Appl Radiat Isot Ano de publicação: 2021 Tipo de documento: Article
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Radioisótopos de Carbono / Compostos Radiofarmacêuticos / Tomografia por Emissão de Pósitrons / Doenças Neuroinflamatórias Limite: Humans Idioma: En Revista: Appl Radiat Isot Ano de publicação: 2021 Tipo de documento: Article