Use of Photosensitizer, Glutaraldehyde, alcohol and Ultrasonics in disinfection of polished and rough surfaces of self-curing polymethyl methacrylate denture base material.

ABSTRACT

AIM: To assess the effectiveness of chemical, ultrasonic and photodynamic therapy (PDT) mediated disinfection against in-vitro biofilms colonized with C. albicans, S. oralis, and S. aureus on surfaces of PMMA (polymethylmethacrylate) denture base material. MATERIALS AND METHODS: Fifty plates of PMMA were prepared with two different surface topography (rough and polished) analyzed by profilometry. Provenance culture (ATCC) of C. albicans, S. oralis, and S. aureus were inoculated on specimens and exposed to disinfection. The disinfection included Rosebengal (RB) 5 µM activated by a red LED, 2% glutaraldehyde, 70% Isopropyl alcohol, ultrasonic disinfection, and saline solution as a control. The antimicrobial efficacy was assessed by subsequently calculating viable counts after disinfection. The data were analyzed using analysis of variance (ANOVA) and Tukey's Post Hoc test. RESULTS: The unpolished surface demonstrated the highest average surface roughness As 5.32±0.36 (µm) and root mean square roughness Rr 7.22±0.81 (µm) compared to the polished surface. Intergroup comparison demonstrated polished and rough PMMA plate disinfected with rose bengal, glutaraldehyde and alcohol-based disinfection exhibited comparable reduction against C. albicans and S. oralis CFU/mL (log10) (p>0.05). Whereas, this reduction was significantly higher on the rough and polished surface of PMMA when disinfected using ultrasounds for 30 sec (p<0.05). Rose Bengal was the most effective method of disinfection against S. aureus both on polished (0.17±0.13) and rough (0.44±0.22) denture plates (p<0.05). CONCLUSION: The ultrasonic disinfection was most effective in reducing viable microbial counts. RB mediated PDT exhibited a more substantial reduction of the colonies of S.aureus.