Cutaneous Regeneration Mechanism of ß-Sheet Silk Fibroin in a Rat Burn Wound Healing Model.

ABSTRACT

Therapeutic dressings to enhance burn wound repair and regeneration are required. Silk fibroin (SF), a natural protein, induces cell migration and serves as a biomaterial in various dressings. SF dressings usually contain α-helices and ß-sheets. The former has been confirmed to improve cell proliferation and migration, but the wound healing effect and related mechanisms of ß-sheet SF remain unclear. We investigated the effects of ß-sheet SF in vivo and in vitro. Alcohol-treated α-helix SF transformed into the ß-sheet form, which promoted granulation formation and re-epithelialization when applied as lyophilized SF dressing (LSFD) in a rat burn model. Our in vitro results showed that ß-sheet SF increased human dermal fibroblast (HDF) migration and promoted the expression of extracellular matrix (ECM) proteins (fibronectin and type III collagen), matrix metalloproteinase-12, and the cell adhesion molecule, integrin ß1, in rat granulation tissue and HDFs. This confirms the role of crosstalk between integrin ß1 and ECM proteins in cell migration. In summary, we demonstrated that ß-sheet SF facilitates tissue regeneration by modulating cell adhesion molecules in dermal fibroblasts. LSFD could find clinical application for burn wound regeneration. Moreover, ß-sheet SF could be combined with anti-inflammatory materials, growth factors, or antibiotics to develop novel dressings.