Simultaneous Analysis of Single-Cell Transcriptomes and Cell Surface Protein Expression of Human Hematopoietic Stem Cells and Progenitors Using the 10x Genomics Platform.

Mende, Nicole; Laurenti, Elisa; Göttgens, Berthold; Wilson, Nicola K

Mende, Nicole; Laurenti, Elisa; Göttgens, Berthold; Wilson, Nicola K.

Afiliação

Mende N; Department of Haematology, Wellcome and MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK.
Laurenti E; Department of Haematology, Wellcome and MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK.
Göttgens B; Department of Haematology, Wellcome and MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK.
Wilson NK; Department of Haematology, Wellcome and MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK. nkw22@cam.ac.uk.

Methods Mol Biol ; 2386: 189-201, 2022.

Article em En | MEDLINE | ID: mdl-34766273

Assuntos

Transcriptoma; Antígenos CD34; Genômica; Células-Tronco Hematopoéticas; Humanos; Imunofenotipagem; Proteínas de Membrana; Análise de Célula Única

Palavras-chave

CD34+ hematopoietic stem and progenitor cells; CITE-seq; Mononuclear cells; Single-cell RNA sequencing; Single-cell proteomics; TotalSeq™

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transcriptoma Limite: Humans Idioma: En Revista: Methods Mol Biol Ano de publicação: 2022 Tipo de documento: Article

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