Your browser doesn't support javascript.
loading
LncRNA FGD5-AS1 functions as an oncogene to upregulate GTPBP4 expression by sponging miR-873-5p in hepatocellular carcinoma.
Zhang, Nuobei; Shen, Hao; Huang, Shenan; Wang, Fenfen; Liu, Huifang; Xie, Fen; Jiang, Lei; Chen, Xin.
Afiliação
  • Zhang N; Department of Gastroenterology, The Second Affiliated Hospital of Nanchang University, Nanchang. znbicx@163.com.
  • Shen H; Department of Gastroenterology, The Second Affiliated Hospital of Nanchang University, Nanchang. 83866161@qq.com.
  • Huang S; Department of Gastroenterology, The Second Affiliated Hospital of Nanchang University, Nanchang. 4417760@qq.com.
  • Wang F; Department of Gastroenterology, The Second Affiliated Hospital of Nanchang University, Nanchang. 553266419@qq.com.
  • Liu H; Department of Gastroenterology, The Second Affiliated Hospital of Nanchang University, Nanchang. 623806969@qq.com.
  • Xie F; Department of Gastroenterology, The Second Affiliated Hospital of Nanchang University, Nanchang. fener87@126.com.
  • Jiang L; Department of Gastroenterology, The Second Affiliated Hospital of Nanchang University, Nanchang. 15083804061@163.com.
  • Chen X; Department of Nuclear Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang. xinchen13870979404@gmx.com.
Eur J Histochem ; 65(4)2021 Nov 16.
Article em En | MEDLINE | ID: mdl-34783233
The long non-coding FGD5-AS1 (LncFGD5-AS1) has been reported to be a novel carcinogenic gene and participant in regulating tumor progression by sponging microRNAs (miRNAs). However, the pattern of expression and the biological role of FGD5-AS1 in hepatocellular carcinoma (HCC) remains largely unknown. The expression level of FGD5-AS1 in tumor tissues and cell lines was measured by RT-qPCR. CCK-8, EdU, flow cytometry, wound healing, and transwell chamber assays were performed to investigate the role of FGD5-AS1 in cell proliferation, apoptosis, migration, and invasion in HCC. Dual luciferase reporter, and RNA pull-down assays were performed to identify the regulatory interactions among FGD5-AS1, miR-873-5p and GTP-binding protein 4 (GTPBP4). We found that the expression of FGD5-AS1 was upregulated in HCC tissues and cell lines. Moreover, the knockdown of FGD5-AS1 suppressed cell proliferation, migration and invasion, and induced apoptosis in HCC cells. Further studies demonstrated that FGD5-AS1 could function as a competitive RNA by sponging miR-873-5p in HCC cells. Moreover, GTPBP4 was identified as direct downstream target of miR-873-5p in HCC cells and FGD5-AS1mediated the effects of GTPBP4 by competitively binding with miR-873-5p. Taken together, this study demonstrated the regulatory role of FGD5-AS1 in the progression of HCC and identified the miR-873-5p/GTPBP4 axis as the direct downstream pathway. It represents a promising novel therapeutic strategy for HCC patients.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oncogenes / RNA Neoplásico / Proteínas Nucleares / Regulação Neoplásica da Expressão Gênica / Regulação para Cima / Carcinoma Hepatocelular / Proteínas de Ligação ao GTP / MicroRNAs / RNA Longo não Codificante / Neoplasias Hepáticas Limite: Humans Idioma: En Revista: Eur J Histochem Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oncogenes / RNA Neoplásico / Proteínas Nucleares / Regulação Neoplásica da Expressão Gênica / Regulação para Cima / Carcinoma Hepatocelular / Proteínas de Ligação ao GTP / MicroRNAs / RNA Longo não Codificante / Neoplasias Hepáticas Limite: Humans Idioma: En Revista: Eur J Histochem Ano de publicação: 2021 Tipo de documento: Article