Simultaneous identification and quantification of amino acids in biofluids by reactive <sup>19</sup>F-tags.

Chen, Ya-Ting; Li, Bin; Li, Xia-Yan; Chen, Jia-Liang; Cui, Chao-Yu; Hu, Kaifeng; Su, Xun-Cheng

Simultaneous identification and quantification of amino acids in biofluids by reactive ¹⁹F-tags.

Chen, Ya-Ting; Li, Bin; Li, Xia-Yan; Chen, Jia-Liang; Cui, Chao-Yu; Hu, Kaifeng; Su, Xun-Cheng.

Afiliação

Chen YT; State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, China. xunchengsu@nankai.edu.cn.
Li B; State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, China. xunchengsu@nankai.edu.cn.
Li XY; State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, China. xunchengsu@nankai.edu.cn.
Chen JL; State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, China. xunchengsu@nankai.edu.cn.
Cui CY; State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, China. xunchengsu@nankai.edu.cn.
Hu K; Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, China. kaifenghu@cdutcm.edu.cn.
Su XC; State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, China. xunchengsu@nankai.edu.cn.

Chem Commun (Camb) ; 57(97): 13154-13157, 2021 Dec 07.

Article em En | MEDLINE | ID: mdl-34812443

ABSTRACT

ABSTRACT

A robust method to identify and quantify amino acids close to physiological conditions by 1D 19F NMR was established. Each 19F-derivatized amino acid has its characteristic chemical-shift profile that is readily identified in the mixture of amino acids or in biofluids including fetal bovine serum and cell lysates. The method shows great potential in metabolomics and biochemical analysis.

Assuntos

Aminoácidos/análise; Líquidos Corporais/química; Escherichia coli/química; Ressonância Magnética Nuclear Biomolecular; Animais; Bovinos; Flúor; Estrutura Molecular

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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Líquidos Corporais / Ressonância Magnética Nuclear Biomolecular / Escherichia coli / Aminoácidos Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Chem Commun (Camb) Ano de publicação: 2021 Tipo de documento: Article

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