Lactylated acidic exopolysaccharide produced by the cyanobacterium Nostoc cf. linckia.
Carbohydr Polym
; 276: 118801, 2022 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-34823807
Cyanobacteria produce a wide range of metabolites of interest for industrial or medical use. The cultivation of freshwater Nostoc cf. linckia yielded 5.4 g/L of a crude exopolysaccharide (cEPS) with a molecular weight of 1.31 × 105 g/mol. Ion-exchange chromatography of cEPS yielded two dominant fractions, EPS-1 and EPS-2, differing in molecular weight. The lower molecular weight fraction (EPS-1) was subjected to structural studies. Results of chemical and spectroscopic analyses showed that three of the four dominant sugars, glucose, galactose and xylose are 1,4-linked in the backbone in the following order: [â4)-ß-D-Xylp-(1 â 4)-ß-D-Glcp-(1 â 4)-α-D-Galp-(1 â 4)-ß-D-Glcp-(1â]n. Terminal mannose residues were identified as side chains linked at C3 of every third backbone xylose and every second glucose is branched at C6 by 3-O-lactyl-ß-D-glucuronic acid (nosturonic acid). Antioxidant properties of EPS were tested using two in vitro methods. Both assays showed that the cEPS was more active than purified EPS-1 and EPS-2 fractions and deproteinized EPS.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos Bacterianos
/
Nostoc
Idioma:
En
Revista:
Carbohydr Polym
Ano de publicação:
2022
Tipo de documento:
Article