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Subcellular localization of nucleocapsid protein of SFTSV and its assembly into the ribonucleoprotein complex with L protein and viral RNA.
Lokupathirage, Sithumini M W; Tsuda, Yoshimi; Ikegame, Kodai; Noda, Kisho; Muthusinghe, Devinda S; Kozawa, Fumiya; Manzoor, Rashid; Shimizu, Kenta; Yoshimatsu, Kumiko.
Afiliação
  • Lokupathirage SMW; Graduate School of Infectious Diseases, Hokkaido University, Sapporo, 060-8638, Japan.
  • Tsuda Y; Department of Microbiology and Immunology, Faculty of Medicine, Hokkaido University, Sapporo, 060-8638, Japan.
  • Ikegame K; Graduate School of Medicine, Hokkaido University, Sapporo, 060-8638, Japan.
  • Noda K; Graduate School of Medicine, Hokkaido University, Sapporo, 060-8638, Japan.
  • Muthusinghe DS; School of Medicine, Hokkaido University, Sapporo, 060-8638, Japan.
  • Kozawa F; Graduate School of Infectious Diseases, Hokkaido University, Sapporo, 060-8638, Japan.
  • Manzoor R; School of Medicine, Hokkaido University, Sapporo, 060-8638, Japan.
  • Shimizu K; International Institute for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan.
  • Yoshimatsu K; Department of Microbiology and Immunology, Faculty of Medicine, Hokkaido University, Sapporo, 060-8638, Japan.
Sci Rep ; 11(1): 22977, 2021 11 26.
Article em En | MEDLINE | ID: mdl-34836987
Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging bunyavirus that causes novel zoonotic diseases in Asian countries including China, Japan, South Korea, and Vietnam. In phleboviruses, viral proteins play a critical role in viral particle formation inside the host cells. Viral glycoproteins (GPs) and RNA-dependent RNA polymerase (RdRp) are colocalized in the Golgi apparatus and endoplasmic reticulum-Golgi intermediate compartment (ERGIC). The nucleocapsid (N) protein was widely expressed in the cytoplasm, even in cells coexpressing GP. However, the role of SFTSV N protein remains unclear. The subcellular localization of SFTSV structural proteins was investigated using a confocal microscope. Subsequently, minigenome and immunoprecipitation assays were carried out. The N protein interacts with viral RNA (vRNA) and further shows translational activity with RdRp which is L protein and localized in the ERGIC and Golgi apparatus when co-expressed with GP. On the other hand, mutant N protein did not interact with vRNA either localized in the ERGIC or Golgi apparatus. The interaction between the N protein of SFTSV and vRNA is important for the localization of viral proteins and viral assembly. This study provides useful insights into the life cycle of SFTSV, which will lead to the detection of antiviral targets.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ribonucleoproteínas / RNA Polimerase Dependente de RNA / RNA Viral / Proteínas do Envelope Viral / Proteínas do Nucleocapsídeo / Retículo Endoplasmático / Febre Grave com Síndrome de Trombocitopenia / Complexo de Golgi Limite: Animals / Humans Idioma: En Revista: Sci Rep Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ribonucleoproteínas / RNA Polimerase Dependente de RNA / RNA Viral / Proteínas do Envelope Viral / Proteínas do Nucleocapsídeo / Retículo Endoplasmático / Febre Grave com Síndrome de Trombocitopenia / Complexo de Golgi Limite: Animals / Humans Idioma: En Revista: Sci Rep Ano de publicação: 2021 Tipo de documento: Article