Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata.
Molecules
; 26(23)2021 Nov 24.
Article
em En
| MEDLINE
| ID: mdl-34885697
ABSTRACT
The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant. O. fimbriata polysaccharide (OFP) was extracted and subsequently purified by chromatography using a DEAE cellulose-52 and Sephadex G-75 column. The molecular weight was determined as 6.2 kDa. HPGPC and monosaccharide composition analysis revealed a homogeneous polysaccharide containing only Glc. Chromatography and spectral analysis showed that the possible chemical structure consisted of â4)-α-Glcp-(1â and a small quantity of â4,6)-ß-Glcp-(1â in the main chain and â6)-ß-Glcp-(1â, α-Glcp-(1â, and ß-Glcp-(1â in the side chain. Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated. OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
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Crassulaceae
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Inflamação
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Anti-Inflamatórios
Limite:
Animals
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Humans
Idioma:
En
Revista:
Molecules
Ano de publicação:
2021
Tipo de documento:
Article