Investigation of sugar binding kinetics of the E. coli sugar/H<sup>+</sup> symporter XylE using solid-supported membrane-based electrophysiology.

Bazzone, Andre; Tesmer, Laura; Kurt, Derya; Kaback, H Ronald; Fendler, Klaus; Madej, M Gregor

Investigation of sugar binding kinetics of the E. coli sugar/H⁺ symporter XylE using solid-supported membrane-based electrophysiology.

Bazzone, Andre; Tesmer, Laura; Kurt, Derya; Kaback, H Ronald; Fendler, Klaus; Madej, M Gregor.

Afiliação

Bazzone A; Department of Biophysical Chemistry, Max-Planck-Institute of Biophysics, Frankfurt/M, Germany. Electronic address: Andre.Bazzone@gmail.com.
Tesmer L; Department of Biophysical Chemistry, Max-Planck-Institute of Biophysics, Frankfurt/M, Germany.
Kurt D; Department of Biophysical Chemistry, Max-Planck-Institute of Biophysics, Frankfurt/M, Germany.
Kaback HR; Department of Physiology and Department of Microbiology, Immunology, Molecular Genetics, University of California, Molecular Biology Institute, Los Angeles, California, USA.
Fendler K; Department of Biophysical Chemistry, Max-Planck-Institute of Biophysics, Frankfurt/M, Germany.
Madej MG; Institute of Biophysics and Biophysical Chemistry, Department of Structural Biology, University of Regensburg, Regensburg, Germany; Institute of Biophysics, Department of Structural Biology, Saarland University, Center of Human and Molecular Biology, Homburg, Germany. Electronic address: Gregor.Made

J Biol Chem ; 298(2): 101505, 2022 02.

Article em En | MEDLINE | ID: mdl-34929170

Assuntos

Proteínas de Escherichia coli; Escherichia coli; Proteínas de Transporte de Monossacarídeos; Simportadores; Metabolismo dos Carboidratos; Eletrofisiologia; Escherichia coli/genética; Escherichia coli/metabolismo; Proteínas de Escherichia coli/metabolismo; Glucose/metabolismo; Cinética; Proteínas de Membrana Transportadoras/genética; Proteínas de Membrana Transportadoras/metabolismo; Proteínas de Transporte de Monossacarídeos/metabolismo; Açúcares/metabolismo; Simportadores/metabolismo

Palavras-chave

MFS; SSM; XylE; bioenergetics; energy coupling; induced fit; membrane transport; sugar transporter

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Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 3_ND Base de dados: MEDLINE Assunto principal: Proteínas de Transporte de Monossacarídeos / Proteínas de Escherichia coli / Simportadores / Escherichia coli Idioma: En Revista: J Biol Chem Ano de publicação: 2022 Tipo de documento: Article

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