MicroRNA126 protects SHSY5Y cells from ischemia/reperfusion injuryinduced apoptosis by inhibiting RAB3IP.
Mol Med Rep
; 25(2)2022 02.
Article
em En
| MEDLINE
| ID: mdl-34935056
ABSTRACT
MicroRNA (miR)126 is known to inhibit inflammatory responses in various inflammatoryrelated diseases, but its role during the cerebral ischemia/reperfusion (I/R) injury remains unknown. The present study aimed to examine the interaction between miR126 and RAB3A interacting protein (RAB3IP), and explore its potential protective effects during I/R injury. The human neuroblastoma cell line SHSY5Y was cultured in an oxygenglucose deprivation/reoxygenation (OGD/R) environment to simulate I/R injury to assess miR126 expression and cell viability. SHSY5Y cells cultured in normal conditions were used as a negative control (NC) group. SHSY5Y cells were transfected with a miR126 mimic or an NC mimic, then cultured in OGD/R conditions; in rescue experiments, SHSY5Y cells were cotransfected with RAB3IP overexpression or NC plasmid together with mimicNC or mimicmiR, and then maintained in an OGD/R environment to evaluate miR126, RAB3IP expression, cell viability and apoptosis. Cell viability was reduced in the Model group compared with the NC group, suggesting the successful construction of the OGD/R model. miR126 expression was downregulated in the Model group compared with the NC group. However, following transfection with mimicmiR, cell viability increased compared with the mimicNC group. Annexin V and PI staining and Hoechst/PI assays also indicated that apoptosis was reduced in the mimicmiR group compared with the mimicNC group. RAB3IP expression was reduced following mimicmiR transfection. In rescue experiments, miR126 negatively regulated RAB3IP expression; by contrast, RAB3IP did not affect that of miR126. In addition, RAB3IP overexpression attenuated the protective effect of miR126 on OGD/Rinduced apoptosis. These findings suggest that miR126 protects against cerebral I/R injury by targeting RAB3IP.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
6_ODS3_enfermedades_notrasmisibles
Base de dados:
MEDLINE
Assunto principal:
Traumatismo por Reperfusão
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Ciclo Celular
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Apoptose
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Fatores de Troca do Nucleotídeo Guanina
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MicroRNAs
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2022
Tipo de documento:
Article