High-Throughput Experimentation Using Cell-Free Protein Synthesis Systems.
Methods Mol Biol
; 2433: 121-134, 2022.
Article
em En
| MEDLINE
| ID: mdl-34985741
ABSTRACT
Cell-free protein synthesis can enable the combinatorial screening of many different components and concentrations. However, manual pipetting methods are unfit to handle many cell-free reactions. Here, we describe a microfluidic method that can generate hundreds of unique submicroliter scale reactions. The method is coupled with a high yield cell-free system that can be applied for broad protein screening assays.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microfluídica
/
Técnicas Analíticas Microfluídicas
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2022
Tipo de documento:
Article