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Characterization and in vitro testing of newly isolated lytic bacteriophages for the biocontrol of Pseudomonas aeruginosa.
Harada, Liliam K; Silva, Erica C; Rossi, Fernando Pn; Cieza, Basilio; Oliveira, Thais J; Pereira, Carla; Tomazetto, Geizecler; Silva, Bianca B; Squina, Fabio M; Vila, Marta Mdc; Setubal, João C; Ha, Taekjip; da Silva, Aline M; Balcão, Victor M.
Afiliação
  • Harada LK; PhageLab - Laboratory of Biofilms & Bacteriophages, University of Sorocaba, Sorocaba/SP, Brazil.
  • Silva EC; PhageLab - Laboratory of Biofilms & Bacteriophages, University of Sorocaba, Sorocaba/SP, Brazil.
  • Rossi FP; Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil.
  • Cieza B; Department of Biophysics & Biophysical Chemistry, Johns Hopkins University, Baltimore, MD, USA.
  • Oliveira TJ; PhageLab - Laboratory of Biofilms & Bacteriophages, University of Sorocaba, Sorocaba/SP, Brazil.
  • Pereira C; Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.
  • Tomazetto G; Department of Engineering, Biological & Chemical Engineering Section (BCE), Aarhus University, Aarhus, Denmark.
  • Silva BB; PhageLab - Laboratory of Biofilms & Bacteriophages, University of Sorocaba, Sorocaba/SP, Brazil.
  • Squina FM; PhageLab - Laboratory of Biofilms & Bacteriophages, University of Sorocaba, Sorocaba/SP, Brazil.
  • Vila MM; PhageLab - Laboratory of Biofilms & Bacteriophages, University of Sorocaba, Sorocaba/SP, Brazil.
  • Setubal JC; Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil.
  • Ha T; Department of Biophysics & Biophysical Chemistry, Johns Hopkins University, Baltimore, MD, USA.
  • da Silva AM; Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil.
  • Balcão VM; PhageLab - Laboratory of Biofilms & Bacteriophages, University of Sorocaba, Sorocaba/SP, Brazil.
Future Microbiol ; 17: 111-141, 2022 01.
Article em En | MEDLINE | ID: mdl-34989245
Aim: Two lytic phages were isolated using P. aeruginosa DSM19880 as host and fully characterized. Materials & methods: Phages were characterized physicochemically, biologically and genomically. Results & conclusion: Host range analysis revealed that the phages also infect some multidrug-resistant (MDR) P. aeruginosa clinical isolates. Increasing MOI from 1 to 1000 significantly increased phage efficiency and retarded bacteria regrowth, but phage ph0034 (reduction of 7.5 log CFU/ml) was more effective than phage ph0031 (reduction of 5.1 log CFU/ml) after 24 h. Both phages belong to Myoviridae family. Genome sequencing of phages ph0031 and ph0034 showed that they do not carry toxin, virulence, antibiotic resistance and integrase genes. The results obtained are highly relevant in the actual context of bacterial resistance to antibiotics.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Bacteriófagos Idioma: En Revista: Future Microbiol Ano de publicação: 2022 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Bacteriófagos Idioma: En Revista: Future Microbiol Ano de publicação: 2022 Tipo de documento: Article