Inhibition of Candida albicans in vivo and in vitro by antimicrobial peptides chromogranin A-N12 through microRNA-155/suppressor of cytokine signaling 1 axis.
Bioengineered
; 13(2): 2513-2524, 2022 02.
Article
em En
| MEDLINE
| ID: mdl-35034584
ABSTRACT
Antimicrobial peptides (AMPs) have proven to inhibit a variety of pathogens. Chromogranin A-N12 (CGA-N12) is a kind of AMP, and it is characterized by stable structure, high anti-Candida activity, and good safety. However, it remains unclear whether CGA-N12 could effectively inhibit the growth of Candida albicans (C. albicans). Colony forming assays were used to measure minimal inhibitory concentration (MIC), minimal fungicidal concentration (MFC), and time-kill curve. Disseminated C. albicans rabbit model was established to investigate the influence of CGA-N12 on histological damage. The protein and mRNA levels of suppressor of cytokine signaling 1 (SOCS1) after treatment were investigated. The MIC and MFC of CGA-N12 against C. albicans was 6 mg/mL. CGA-N12 considerably inhibited germ tube formation of C. albicans. The fungal load in the tissues and inflammatory factors in the serum were suppressed by CGA-N12. CGA-N12 significantly reduced the histological changes caused by C. albicans, and the protein and mRNA levels of SOCS1 were markedly inhibited. The inhibition effect of CGA-N12 on C. albicans and significant improvement of histological damage by CGA-N12 through microRNA-155/SOCS1 axis were proved in this study. This study proposes a novel therapeutic strategy for the treatment and prevention of C. albicans.Abbreviations AMPs Antimicrobial peptides; MIC Minimal inhibitory concentration; MFC Minimal fungicidal concentration; AIDS Acquired immune deficiency syndrome; PBS Phosphate buffer saline; FBS Fetal bovine serum; ROS Reactive oxygen species; CFU Colony formation unit; CGA Chromogranin A; SOCS1 Suppressor of cytokine signaling 1; SDA Sabouraud Dextrose Agar; GRAVY Grand average of hydropathicity; C. parapsilosis Candida parapsilosis; C. albicans Candida albicans.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Candida albicans
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Candidíase
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Transdução de Sinais
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MicroRNAs
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Cromogranina A
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Proteína 1 Supressora da Sinalização de Citocina
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Peptídeos Antimicrobianos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Bioengineered
Ano de publicação:
2022
Tipo de documento:
Article