Assessing Oligomerization Status of Mitochondrial OXPHOS Complexes Via Blue Native Page.
Methods Mol Biol
; 2413: 55-62, 2022.
Article
em En
| MEDLINE
| ID: mdl-35044654
ABSTRACT
Mitochondrial metabolism plays key roles in pathologies such as cancer. The five complexes of the oxidative phosphorylation (OXPHOS) system are crucial for producing ATP and maintaining cellular functions and are particularly exploited in cancer cells. Understanding the oligomeric state of these OXPHOS complexes will help elucidate their function (or dysfunction) in cancer cells and can be used as a mechanistic tool for anticancer agents that target mitochondria. Here we describe a protocol to observe the oligomeric state of the five OXPHOS complexes by isolating mitochondrial-enriched fractions followed by assessing their oligomeric state by nondenaturing blue native page electrophoresis.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosforilação Oxidativa
/
Mitocôndrias
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2022
Tipo de documento:
Article