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Characterization of pain-related behaviors, changes in bone microarchitecture and sensory innervation induced by chronic cadmium exposure in adult mice.
Torres-Rodríguez, Héctor F; Graniel-Amador, Mayra A; Cruz-Camacho, Cindy J; Cantú-Martínez, Alma A; Martínez-Martínez, Arisai; Petricevich, Vera L; Montes, Sergio; Castañeda-Corral, Gabriela; Jiménez-Andrade, Juan M.
Afiliação
  • Torres-Rodríguez HF; Laboratorio de Farmacología, Unidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, Mexico.
  • Graniel-Amador MA; Laboratorio de Farmacología, Unidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, Mexico.
  • Cruz-Camacho CJ; Laboratorio de Farmacología, Unidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, Mexico.
  • Cantú-Martínez AA; Laboratorio de Farmacología, Unidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, Mexico.
  • Martínez-Martínez A; Laboratorio de Farmacología, Unidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, Mexico.
  • Petricevich VL; Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
  • Montes S; Laboratorio de Farmacología, Unidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, Mexico.
  • Castañeda-Corral G; Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
  • Jiménez-Andrade JM; Laboratorio de Farmacología, Unidad Académica Multidisciplinaria Reynosa-Aztlán, UAT, Reynosa, Tamaulipas, Mexico. Electronic address: jimenez.andrade1810@gmail.com.
Neurotoxicology ; 89: 99-109, 2022 03.
Article em En | MEDLINE | ID: mdl-35065951
Because of the relative lack of understanding of the neurobiological mechanisms that drive toxic effects of cadmium in bone, the purpose of this study was to characterize a preclinical model of chronic cadmium exposure. Adult male C57BL/6 J mice were exposed to cadmium 25 mg/L (as CdCl2) in drinking water for 16 weeks. During this time, pain-related behaviors including hindpaw mechanical sensitivity and vertical rears were evaluated every four weeks. We assessed changes in bone microarchitecture at the femoral neck and L5 vertebra by microcomputed tomography and quantified the density of nerve fibers expressing PGP 9.5 (a pan-neuronal marker) and CGRP (a marker of sensory nerve fibers subfamily) at the femoral neck and glabrous skin of the hindpaw using immunohistochemistry. Cadmium exposure produced mechanical hypersensitivity in both hindpaws along with decreased rearing activity (surrogate for musculoskeletal-related pain) without affecting the horizontal activity (a measure of locomotor behavior) in comparison to the control group. Intraperitoneal acute treatment with morphine and gabapentin reversed pain-related behaviors in cadmium-exposed mice. Furthermore, exposure to cadmium resulted in significant trabecular bone deterioration at the femoral neck and L5 vertebra. We also observed a significant reduction in the density of both CGRP+ and PGP 9.5+ nerve fibers in the femoral neck, but not in the hindpaw glabrous skin, suggesting tissue-dependent neurotoxicity. This model may help in developing a mechanism-based understanding of the factors that generate and maintain musculoskeletal pain and bone loss caused by chronic cadmium exposure and in translating these findings into new therapies for treating cadmium-induced bone toxicity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cádmio / Colo do Fêmur Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Neurotoxicology Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cádmio / Colo do Fêmur Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Neurotoxicology Ano de publicação: 2022 Tipo de documento: Article