Evaluation of a <sub>D</sub>-Octaarginine-linked polymer as a transfection tool for transient and stable transgene expression in human and murine cell lines.

Sakuma, Saki; Okamoto, Mariko; Matsushita, Nao; Ukawa, Masami; Tomono, Takumi; Kawamoto, Keiko; Ikeda, Teruo; Sakuma, Shinji

Evaluation of a _D-Octaarginine-linked polymer as a transfection tool for transient and stable transgene expression in human and murine cell lines.

Sakuma, Saki; Okamoto, Mariko; Matsushita, Nao; Ukawa, Masami; Tomono, Takumi; Kawamoto, Keiko; Ikeda, Teruo; Sakuma, Shinji.

Afiliação

Sakuma S; Laboratory of Immunology and Infection Control, Department of Veterinary Medicine, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Okamoto M; Present address: Division of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki, Japan.
Matsushita N; Laboratory of Immunology and Infection Control, Department of Veterinary Medicine, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Ukawa M; Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Tomono T; Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Kawamoto K; Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Ikeda T; Laboratory of Immunology and Infection Control, Department of Veterinary Medicine, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Sakuma S; Laboratory of Immunology and Infection Control, Department of Veterinary Medicine, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.

J Vet Med Sci ; 84(4): 484-493, 2022 Apr 13.

Article em En | MEDLINE | ID: mdl-35135938

Assuntos

DNA; Polímeros; Animais; Linhagem Celular; Proteínas de Fluorescência Verde/genética; Proteínas de Fluorescência Verde/metabolismo; Humanos; Camundongos; Oligopeptídeos; Plasmídeos/genética; Transfecção/veterinária; Transgenes

Palavras-chave

gene-delivery; plasmid-derived protein expression; poly(N-vinylacetamide-co-acrylic acid) bearing d-octaarginine; stable transgene expression; transient transgene expression

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polímeros / DNA Limite: Animals / Humans Idioma: En Revista: J Vet Med Sci Ano de publicação: 2022 Tipo de documento: Article

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